Early-Mid Career Cytometrist Session
Wednesday, August 30, 2023 |
10:50 AM - 12:30 PM |
Overview
Chair: Dr Anna Brooks & Dr Emily Edwards
Early-Mid Career Cytometrist Speakers: Dr Reuben McGregor, Thaize Chometon, Tanvi Damani, Brad Devery, Dr Kathryn Hally, Justin Tirados, Ash Sargent, Rebecca Castle, Nicole Bacalhau , Kirstie Theodore
Early-Mid Career Cytometrist Speakers: Dr Reuben McGregor, Thaize Chometon, Tanvi Damani, Brad Devery, Dr Kathryn Hally, Justin Tirados, Ash Sargent, Rebecca Castle, Nicole Bacalhau , Kirstie Theodore
Speaker
Dr Reuben McGregor
Research Fellow
University of Auckland
The START Study: Immune profiling to illuminate rheumatic fever pathogenesis
10:50 AM - 11:00 AMBiography
Ms Thaize Chometon
Flow Cytometry Technologist
University of Auckland
30+ colour full spectrum panel using a 3 laser instrument for immunophenotyping human Whole Blood and peripheral blood mononuclear cells
11:00 AM - 11:10 AMBiography
Thaize Chometon is Flow Cytometry Technologist at Auckland Cytometry, University of Auckland. Currently working in complex multi-colour panel design and optimization, she excels in build up to 40+ colours panels using spectral cytometry while eagerly embracing new challenges and projects. Thaize's role extends to training and supporting facility users, troubleshooting experimental design, cell sorting and data analysis. With seven years of experience in the field, including three years at the Oswaldo Cruz Foundation in Brazil, Thaize has been an integral part of the Auckland Cytometry team since 2019, contributing to advancements in flow cytometry research.
Ms Tanvi Damani
Assistant Technologist
University of Auckland
Comparative Analysis: Assessing the Efficacy of an Automated Wash System vs. Conventional Methods for Flow Cytometry Staininge
11:10 AM - 11:20 AMBiography
Tanvi Damani serves as an Assistant Technologist at Auckland Cytometry, located within the University of Auckland. In her position, Tanvi is responsible for providing training, education, and assistance to researchers utilizing the flow cytometry facility.
Tanvi's involvement in the field of flow cytometry originated during her time as a Master's student at the University of Auckland, where she focused on cell sorting. Throughout the years, her experience expanded to encompass conducting flow experiments for clinical trials. Eventually, she assumed the role of Technologist at Auckland Cytometry.
Mr Brad Devery
Phd Candidate
University of Otago
High-dimensional analysis reveals myeloid cell heterogeneity and plasticity in CRC patient samples.
11:20 AM - 11:30 AMBiography
Brad completed his Bachelor of Science in 2021, before joining the Kemp Lab at the University of Otago as an Honours student.
Here he investigated the role of heterogeneity in colorectal cancer where he incorporated a kaupapa Māori lens. The first to do so in the School of Biomedical Sciences.
Brad commenced his PhD in 2023 under the primary supervision of Professor Roslyn Kemp and is co-supervised by Dr. Kirsten Ward Harstonge and Karyn Paringatai.
His research interests include high-dimensional analysis, kaupapa Māori approaches and the role of myeloid cells in anti-tumour responses.
Dr Kathryn Hally
Lecturer
University of Otago
Extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) modulate primary human macrophage phenotype in vitro.
11:30 AM - 11:40 AMBiography
Dr Kathryn Hally is a lecturer at the University of Otago in Wellington. Her current research focuses on developing inflammation-based risk scores for predicting optimal myocardial repair in patients with acute myocardial infarction. Among other techniques, Kathryn uses flow cytometry to interrogate circulating immune cell phenotype for biomarker discovery in cardiovascular disease.
Mr Justin Tirados
Phd Student
University of Otago
Effect of canonical WNT signalling on effector regulatory T cell formation and function
11:40 AM - 11:50 AMBiography
Justin gained a Bachelor of Science majoring in Human Biology at the De La Salle University in Manila, Philippines. He then proceeded to study towards a Doctor of Medicine degree but towards the end of the training realised that the profession was not for him. He then went to the University of Otago and gained a Bachelor of Biomedical Sciences majoring in Infection and Immunity. He proceeded to do a Bachelor of Biomedical Sciences Honours degree majoring in Infection and Immunity. He is currently working towards getting his PhD. He utilises spectral flow cytometry technique in his PhD project.
Mr Ashly Sargent
Phd Student
University of Auckland
SARS-CoV-2 spike peptide pool responsiveness in triple vaccinated and chronically immunosuppressed transplant patients within the CoVac cohort.
11:50 AM - 12:00 PMBiography
Ash Sargent is a second-year PhD student at the University of Auckland under Professor Rod Dunbar.
Primarily focused on T cell immunology with a particular interest in clinical translation and research and a little over four years of experience in the use of flow cytometry with experience in cell sorting (BD FACS Aria II, CytoFLEX SRT cell sorter) and spectral flow cytometry development using the Cytek Aurora and Northern lights instruments.
Miss Rebecca Castle
Research Assistant
Peter MacCallum Cancer Centre
Why use mass cytometry to analyse the immune microenvironment in renal cell carcinoma (RCC).
12:00 PM - 12:10 PMBiography
Rebecca Castle is an early career research assistant primarily focusing on using mass cytometry in describing the immune changes that occur within cancer patients on clinical trials. She’s worked on translational research on multiple clinical trials within the past 18 months and has since learnt the benefits of using mass cytometry in comparison to flow cytometry. She is currently working on the translational research component of a clinical trial in renal cell carcinoma, investigating the effects of a new combination radiotherapy on the immune context both locally and systemically.
Miss Nicole Bacalhau
Hospital Scientist
The Children’s Hospital at Westmead
Use of the respiratory burst assay after bone marrow transplant for children with chronic granulomatous disease.
12:10 PM - 12:20 PMBiography
Nicole is a Hospital Scientist at The Westmead Children's Hospital in Sydney within the Immunology and Allergy Department. With 9 years of experience in flow cytometry, she previously worked with Dr. Alberto Orfao's team at CIC, Salamanca, Spain. Currently, Nicole is a fellowship trainee for the Faculty of Science at RCPA.
Ms Kristie Theodore
Scientist
Pathology Queensland
Extrathymic cortical thymocytes in infant adenoidal tissue.
12:20 PM - 12:30 PMBiography
Kristie’s scientific career began in 2014 in private pathology where she had her first encounter with flow cytometry.
When a position became available in 2015 in the Autoimmune-Immunobiology Section of Pathology Queensland, she jumped at the chance to further develop her keen interest in clinical flow cytometry.
Although relatively new to the field, Kristie has had the opportunity to work on various projects within the laboratory including the validation of instrumentation and the development of new methods with a special interest in primary immunodeficiency diagnosis.
Outside of work, Kristie loves travelling, listening to true crime podcasts and eating pasta.
Chair
Anna Brooks
Senior Lecturer
University of Auckland
Judge
Suat Dervish
Core Facility Manager
WIMR
Joanna Roberts
Scientist/director
Flowjoanna
Robert Salomon
Operations and Technology Manager
CCIA